Biolegend, 306007, PE/Cyanine5 anti-human CD123 Antibody
25tests
Catalog Number: 306007
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Category
Manufacturer's Pagewww.biolegend.com/en-us/products/pe-cyanine5-anti-human-cd123-antibody-2858
Description
CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.
Specifications
General
| Verified Reactivity | Human |
|---|---|
| Reported Reactivity | Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human IL-3Rα transfected COS cells. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions. |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. |
| Excitation Laser | Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
| Application Notes | Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7. |