Biolegend, 307828, APC/Cyanine7 anti-human IgD Antibody
100tests
Catalog Number: 307828
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Category
Description
IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell. (BioLegend Website)
Specifications
General
| Verified Reactivity | Human |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Native human IgD protein (full length) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Excitation Laser | Red Laser (633 nm) |
| Application Notes | This clone does not cross react with mouse tissues. |
| RRID | AB_3106084 (BioLegend Cat. No. 307827) AB_3106084 (BioLegend Cat. No. 307828) |
| Distribution | Naïve B cells |
| Function | Antigen binding, B cell activation |
| Cell Type | B cells |
| Biology Area | Immunology |
| Antigen References | Geisberger R, et al. 2006. Immunology. 118:429-37. Weller S, et al. 2005. Eur J Immunol. 35:2789-92. Brandtzaeg P & Johansen FE. 2005. Immunol Rev. 206:32-63. |
| Gene ID | 3495 |
| UniProt | View information about IgD on UniProt.org |
| Clone | W18340F |
| Regulatory Status | RUO |
| Other Names | Ig delta chain C region |
| Isotype | Rat IgG2a, κ |
