Biolegend, 322011, Ultra-LEAF™ Purified anti-human Lymphotoxin β Receptor (LTβR) Antibody
100microg
Catalog Number: 322011
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human LT-βR Fc protein |
| Formulation | 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. |
| Endotoxin Level | Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test. |
| Preparation | The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography. |
| Concentration | The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions. |
| Application | FC - Quality tested WB, IP, Apop - Reported in the literature, not verified in house |
| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes | Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, ELISA detection1, induction of apoptosis2. The biotinylated 31G4D8 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with the purified 36G9G3 antibody (Cat. No. 322101/322102) as capture antibody. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 322003/322004). |
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| Application References(PubMed link indicates BioLegend citation) | Developer's communication Chen M-C, et al. 2003. J. Biol. Chem. 278:16073. |
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| RRID | AB_2832630 (BioLegend Cat. No. 322011) AB_2832631 (BioLegend Cat. No. 322012) |
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| Structure | Member of the TNF receptor superfamily; transmembrane receptor containing 3 TNF receptor domains, predicted molecular weight approximately 47 kD |
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| Distribution | Highly expressed in lung, liver, and kidney. Moderate expression in heart and testes; weak expression in brain, thymus, spleen, and lymph nodes. |
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| Function | Promotes apoptosis via TRAF3 and TRAF5, plays a role in the development of lymphoid organs |
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| Interaction | Receptor for heterotrimeric lymphotoxin complex (composed of LTα and LTβ as well as LIGHT. Also interacts with TRAF3, TRAF4, and TRAF5 as well as hepatitis C virus core protein |
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| Cell Type | Embryonic Stem Cells |
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| Biology Area | Immunology, Stem Cells |
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| Molecular Family | Cytokine/Chemokine Receptors |
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| Antigen References | Crowe PD, et al. 1994 Science 264:707. |
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| Gene ID | 4055 |
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| UniProt | View information about LTbetaR on UniProt.org |
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| Clone | 31G4D8 |
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| Regulatory Status | RUO |
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| Other Names | Lymphotoxin beta receptor, Tumor necrosis factor receptor superfamily member 3 |
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| Isotype | Mouse IgG2b, κ |
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