Biolegend, 325502, Purified anti-human IgE Antibody
100microg
Catalog Number: 325502
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human Ig cocktail |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | FC - Quality tested ELISA, IHC - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes | The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis. |
| Application References(PubMed link indicates BioLegend citation) |
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| Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed. |
| Product Citations | Balemans D,et al. 2017. Sci Rep. . 10.1038/s41598-017-12618-7. PubMed Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed Maehara T, et al. 2019. Medicine (Baltimore). 98:e18300. PubMed Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Cildag S, et al. 2021. Med Pharm Rep. 94:53. PubMed |
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| RRID | AB_830847 (BioLegend Cat. No. 325502) |
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| Distribution | B cells |
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| Cell Type | B cells |
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| Biology Area | Immunology |
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| Gene ID | 3497 |
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| UniProt | View information about IgE on UniProt.org |
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| Clone | MHE-18 |
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| Regulatory Status | RUO |
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| Other Names | Immunoglobulin E |
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| Isotype | Mouse IgG1, κ |
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