Biolegend, 331908, PE anti-human CD335 (NKp46) Antibody
100tests
Catalog Number: 331908
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | NKp46-Fc fusion protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. |
| Excitation Laser | Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
| Application Notes | Clone 9E2 has been shown to block NK activation through NKp46.6 |
| Application References(PubMed link indicates BioLegend citation) | Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309. Kalberer CP, et al. 2003. Blood 102:127. Chen Y, et al. 2007. J. Immunol. 179:2766. Jarahian M, et al. 2009. J. Virol. 83:8108. PubMed Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Achdout H. et al. 2010. J. Virol. 84:3993. |
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| Product Citations | Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed Merah-Mourah F, et al. 2021. Curr Protoc. 1:e229. PubMed Lee Y, et al. 2020. J Innate Immun. 1:. PubMed Mayassi T, et al. 2019. Cell. 176:967. PubMed Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed Spallanzani R, et al. 2015. J Immunol. 195: 2141-2148. PubMed Rademacher MJ, et al. 2021. Sci Rep. 11:8321. PubMed Rajashekar JK, et al. 2021. Cell Host Microbe. 29(6):904-916.e6. PubMed Draganov DD, et al. 2019. J Transl Med. 17:100. PubMed Ponath V, et al. 2021. Int J Mol Sci. 22:. PubMed Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed Samarani S, et al. 2020. Mediators Inflamm. 2020:6401969. PubMed Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed Collison J, et al. 2015. J Immunol. 195: 1162-1170. PubMed Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed Sadallah S, et al. 2016. J Immunol. 197: 1663 - 1671. PubMed Zanetti S, et al. 2016. J Biol Chem. 291: 16541 - 16552. PubMed |
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| RRID | AB_1027679 (BioLegend Cat. No. 331907) AB_1027666 (BioLegend Cat. No. 331908) |
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| Structure | Type I membrane glycoprotein (46 kD) |
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| Distribution | Expressed on resting and activated NK cells |
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| Cell Type | NK cells |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147. 2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309. 3. Sivori S. 1999. Eur. J. Immunol. 29:1656. |
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| Gene ID | 9437 |
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| UniProt | View information about CD335 on UniProt.org |
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| Clone | 9E2 |
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| Regulatory Status | RUO |
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| Other Names | NKp46, NCR1 |
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| Isotype | Mouse IgG1, κ |
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