Biolegend, 400289, Ultra-LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl Antibody
50mg
Catalog Number: 400289
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| Antibody Type | Monoclonal |
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| Host Species | Mouse |
| Formulation | 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. |
| Endotoxin Level | Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test. |
| Preparation | The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography. |
| Concentration | The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions. |
| Application | FC - Quality testedICFC, ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. |
| Application Notes | Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA). |
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| Product Citations | Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed van der Sluis RM, et al. 2022. EMBO J. 41:e109622. PubMed Montes de Oca M, et al. 2016. Cell Rep. 17:399-412. PubMed Sugimoto C, et al. 2022. Elife. 11:. PubMed Abe S, et al. 2020. JCI Insight. 5:00. PubMed Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed Lee Y, et al. 2022. Front Immunol. 12:780210. PubMed Langer V, et al. 2019. J Clin Invest. 129:4691. PubMed Arakawa A, et al. 2021. J Immunol. 207:2235. PubMed Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed Kaur A, et al. 2016. Nature. 532: 250-254. PubMed Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed Weckbach LT, et al. 2019. J Exp Med. 216:350. PubMed |
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| RRID | AB_11149166 (BioLegend Cat. No. 400263) AB_11148947 (BioLegend Cat. No. 400264) AB_11148947 (BioLegend Cat. No. 400281) AB_11148947 (BioLegend Cat. No. 400282) AB_11148947 (BioLegend Cat. No. 400289) AB_11148947 (BioLegend Cat. No. 400290) |
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| Gene ID | NA |
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| Clone | MOPC-173 |
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| Regulatory Status | RUO |
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| Isotype | Mouse IgG2a, κ |
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