Description
The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Specifications

General

Antibody TypeMonoclonal
Host SpeciesMouse
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
PreparationThe antibody was purified by affinity chromatography and conjugated with Spark Violet™ 500 under optimal conditions.
Concentration0.5 mg/mL
Storage & HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ApplicationFC - Quality tested
Recommended UsageEach lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product. * Spark Violet™ 500 has a maximum excitation of 393 nm and a maximum emission of 500 nm.
Excitation LaserViolet Laser (405 nm)
Application NotesAdditional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
Application References(PubMed link indicates BioLegend citation)Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA) Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF) Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Lian IA, et al. 2011. Placenta. 32:823. PubMed Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
RRIDAB_3097150 (BioLegend Cat. No. 402425) AB_3097150 (BioLegend Cat. No. 402426)
Gene IDNA
CloneMOPC-21
Regulatory StatusRUO
IsotypeMouse IgG1, κ