| Verified Reactivity | Mouse |
|---|---|
| Antibody Type | Polyclonal |
| Host Species | Goat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions. |
| Concentration | 0.2 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage |
| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. |
| Excitation Laser | Violet Laser (405 nm) |
|---|
| Application Notes | This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA. |
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| Application References(PubMed link indicates BioLegend citation) | Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70. |
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| Product Citations | Clifton C, et al. 2022. Front Cell Infect Microbiol. 12:1035566. PubMed |
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| RRID | AB_2616858 (BioLegend Cat. No. 405331) |
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| Distribution | B cells |
|---|
| Gene ID | 16059 |
|---|
| UniProt | View information about IgG on UniProt.org |
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| Clone | Poly4053 |
|---|
| Regulatory Status | RUO |
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| Isotype | Goat Polyclonal IgG |
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