Biolegend, 511802, Purified anti-mouse IL-12 (p70) Antibody
500microg
Catalog Number: 511802
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | CHO expressed, recombinant mouse IL-12 p70 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | ELISA Capture |
| Recommended Usage | Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12(p70) recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes | ELISA Capture: The purified C18.2 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with the biotinylated C17.8 antibody as detection antibody and recombinant mouse IL-12 p70 as the standard. |
| Product Citations |
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| Volpedo G, et al. 2022. NPJ Vaccines. 7:32. PubMed Liang D, et al. 2018. Front Immunol. 8:1801. PubMed Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed Jin X, et al. 2020. Cell Reports. 31(8):107690. PubMed Zhou Y, et al. 2018. Cell Mol Immunol. 15:246. PubMed Liu C, et al. 2019. Theranostics. 9:1369. PubMed Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed |
| RRID | AB_2123771 (BioLegend Cat. No. 511801) AB_2123769 (BioLegend Cat. No. 511802) |
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| Structure | heterodimeric cytokine composed of p40 subunit shared with IL-23 and a specific p35 subunit |
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| Distribution | Secreted by dendritic cells, monocytes/macrophages, B cells, T cells |
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| Function | Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23 |
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| Interaction | T cells, NK cells |
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| Ligand/Receptor | IL-12Rβ1 binds p40 shared with IL-23; dimeric with IL-12Rβ2 binds p35 |
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| Bioactivity | IL-12 p70 (p40:p35) induces IFN-γ, TNF-α production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. |
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| Cell Type | Dendritic cells, Monocytes, Macrophages, B cells, T cells |
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| Biology Area | Immunology |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Lankford CS, et al. 2003 J. Leukoc. Biol.. 73:49.2. Parham C, et al. 2002 J. Immunol.. 168:5699.3. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.5. Quesniaux V. 1992. Research Immunol. 143:385.6. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355. |
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| Gene ID | 1615916160 |
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| UniProt | View information about IL-12 p70 on UniProt.org |
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| Clone | C18.2 |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-12 p70, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF) |
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| Isotype | Rat IgG2a, κ |
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