Biolegend, 559206, Recombinant Human TNFSF18 (GITRL) (carrier-free)
100microg
Catalog Number: 559206
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Category
Manufacturer's Pagewww.biolegend.com/en-us/products/recombinant-human-tnfsf18-gitrl-carrier-free-10819
Description
TNFSF18 is a type II transmembrane glycoprotein that belongs to the TNF superfamily. This protein is mainly expressed on antigen presenting cells (APC) including macrophages, B cells, dendritic cells (DCs), and endothelial cells. Its expression increases upon the stimulation of proinflammatory stimuli. The receptor of TNFSF18 is the glucocorticoid-induced TNFR-related protein (GITR), which is a member of the TNFR superfamily. Engagement of TNFSF18 and GITR leads to T cell activation, proliferation, and cytokine production. It also inhibits the suppressive function of T regulatory cells and induces macrophage activation. GITR-TNFSF18 interaction plays an essential role in the regulation of the immune response to self antigens and pathogens. In addition, this receptor-ligand interaction has been shown to modulate anti-tumor immunity.
Specifications
General
| Source | Human TNFSF18, amino acids (Glu74-Ser199) (Accession# NP_005083), was expressed in 293E cells with an N-terminal His tag. |
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| Molecular Mass | The 147 amino acid recombinant protein has a predicted molecular mass of approximately 16.5 kD. The protein migrates at about 25 kD in DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is His. |
| Purity | >95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS, pH 7.2. |
| Endotoxin Level | Less than 1.0 EU per µg of protein as determined by the LAL method. |
| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |