Biolegend, 570508, Recombinant Human IL-17A (carrier-free)

Name: Recombinant Human IL-17A (carrier-free)
Brand: Biolegend
SKU: 570508

500microg

Catalog Number: 570508

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Category

Amino Acids

Manufacturer's Pagewww.biolegend.com/en-us/products/recombinant-human-il-17a-carrier-free-4566

Description

IL-17A was initially identified from a subtracted cDNA library between closely related murine lymphoid cells and called CTLA-8, and share 58% homology with an open reading frame of the T-lymphotropic Herpesvirus Samirii virus (viral IL-17) (5). IL-17A belongs to a family of cytokines, which has five members; designated IL-17A-F. IL-17 is expressed by a unique lineage of CD4 T cells (Th17) that develop in response to IL-23, in particular under conditions in which Th1 and Th2 development are suppressed. IL-17A shares the greatest homology (55%) with IL-17F. Both IL-17A and IL-17F are produced by Th17 cells. IL-17A and IL-17F can either exist as IL-17A homodimers and IL-17F homodimers or as IL-17A-IL-17F heterodimers (6). IL-17 is a key mediator of autoimmune disorders, including rheumatoid arthritis, psoriasis, inflammatory bowel disease, and asthma, and plays a role in host defense (7).

Specifications

General

Source	Human IL-17A, amino acids Ile20-Ala155 (Accession # NM_002190) was expressed in E. coli.
Molecular Mass	The 137 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 15,666 Da. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 16kDa by SDS-PAGE. The non-reduced protein migrates as a homodimer, at approximately 28kDa by SDS-PAGE.
Purity	Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation	0.22 µm filtered protein solution is in 10 mM Sodium Phosphate buffer, 300 mM NaCl, pH 7.2.
Endotoxin Level	Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Concentration	10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.