Biolegend, 577002, Recombinant Mouse IL-12 (p70) (carrier-free)
10microg
Catalog Number: 577002
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| Source | Expressed in Sf9 cells as secreted protein (p40: Accession# NM_008352, p35: Accession#NM_008351). |
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| Molecular Mass | The mIL-12 consists of two subunits linked via a disulphide bond: P40 (Accession# P43432: Met23-Ser335) and P35 (Accession# NP_032377: Arg23-Ala215). The total predicted molecular weight is 57.48 kD. The non-reduced protein migrates at approximately 55 kD and the DTT-reduced protein produces two bands at approximately 40 kD and 24 kD by SDS-PAGE. |
| Purity | 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in 10 mM Sodium Phosphate buffer, 150 mM NaCl, pH 7.2. |
| Endotoxin Level | Less than 0.01 ng per µg cytokine as determined by the LAL method. |
| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | Mouse IL-12 induces INFγ production in mouse splenocytes in a dose dependent manner. The ED50 = 0.01 to 0.1 ng/ml. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Additional Product Notes | Get a 50% discount on this product when purchased in our Activation Bundles. Restrictions apply. Learn more… |
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| Product Citations | Pandey SP, et al. 2022. Cell Host Microbe. 30:1003. PubMed Aso K, et al. 2023. Nat Commun. 14:984. PubMed Arkatkar T, et al. 2023. J Clin Invest. 133:. PubMed Fukuhara H, et al. 2023. Commun Med (Lond). 3:40. PubMed Vereertbrugghen A, et al. 2023. J Neuroinflammation. 20:120. PubMed Ferreira ACF, et al. 2023. Immunity. 1468:56. PubMed Hsieh T, et al. 2022. Front Immunol. 13:901030. PubMed Balood M, et al. 2022. Nature. 611:405. PubMed Benakis C, et al. 2022. Elife. 11: . PubMed Ye L, et al. 2022. Cell Metab. 34:595. PubMed Hsu TS, et al. 2020. Nat Commun. 3.934027778. PubMed Yu Y, et al. 2022. iScience. 25:105004. PubMed Di Modica M, et al. 2021. Cancer Res. 81:2195. PubMed Gomez–Lopez N, et al. 2019. J Immunol. 203:1793. PubMed Huang A, et al. 2019. JCI Insight. 4. PubMed Montes de Oca M, et al. 2020. PLoS Pathog. 16:e1008994. PubMed Xu Y, et al. 2019. JCI Insight. 4:5. PubMed Bremser A, et al. 2015. PLoS One. 10: 0137393. PubMed Ramstead AG, et al. 2020. Cell Rep. 30:2889. PubMed Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed Swan G, et al. 2021. Front Immunol. 12:641188. PubMed Li N, et al. 2021. iScience. 24:103163. PubMed Everts B, et al. 2016. J Exp Med. 213: 35 - 51. PubMed Mohammadpour H, et al. 2020. JCI Insight. 5:00. PubMed Luo X, et al. 2021. Neuron. 109:2691. PubMed Bao K, et al. 2016. J Immunol. 197(11):4371-4381. PubMed Yue T, et al. 2022. Cell Death Differ. 29:218. PubMed Xu K, et al. 2021. STAR Protoc. 2:100757. PubMed |
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| Structure | Heterodimer |
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| Function | IL-12 is produced by myeloid cells and DCs in response to microbial stimuli, such as those mediated by bacteria, fungi, viruses, and intracellular parasites. IL-12 drives Th1 differentiation and IFNγ production. IL-12 acts as a bridge between innate resistance and adaptive immunity. IL-12 production by activated APC is suppressed by IL-10. In addition, IL-12 production by macrophages is regulated by TNFα and nitric oxide. TLR-4 and TLR-9 can cooperate to increase selectively IL-12 production by murine dendritic cells. |
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| Interaction | Cells of hematopoietic origin express the IL-12R, including NK cells, activated T-cells and dendritic cells. |
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| Ligand/Receptor | IL-12 receptor is a heterodimer containing IL-12Rβ1 and IL-12Rβ2 subunits. |
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| Cell Sources | IL-12 is produced by monocytes, macrophages, neutrophils, dendritic cells and B cells. In the CNS, astrocytes and microglia are the main sources of IL-12. |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Schoenhaut DS, et al. 1992. J. Immunol.. 148:3433. 2. Manetti R, et al. 1994. J. Exp. Med. 179:1273. 3. Ireland D, et al.2005. Viral Immunol. 18:397. 4. Moreno SE, et al. 2006. J. Immunol. 177:3218. 5. Lyakh L, et al. 2008. Immunol. Rev.. 226:112. 6. Theiner G, et al. 2008. Mol. Immunol. 45:244. |
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| Gene ID | 1615916160 |
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| UniProt | View information about IL-12 p70 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF). |
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