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Description
Mouse IL-34 shares a sequence identity of 71% with human IL-34 on the amino acid level, and the IL-34 gene is syntenic in the human, chimpanzee, rat, and mouse genomes. IL-34 has no apparent consensus structural domain or motif and does not share sequence homology with M-CSF; nevertheless, it binds to the CSFR. These two cytokines are not identical in biological activity and signal activation. IL-34 and CSF show an equivalent ability to support cell growth or survival. However, these cytokines have different ability to induce the production of chemokines (MCP-1 and eotaxin-2) in primary macrophages, the morphological change in TF-1-fms cells, and the migration of J774A.1 cells. The use of monoclonal antibodies against the CSFR suggests a differential domain binding in the receptor to IL-34 and CSF. As a result, different bioactivities and signal activation kinetics/strength are produced for these cytokines.
Specifications

General

SourceThe mouse IL-34 (Accession# NM_001135100.1: Asn21 - Pro235 with a C-terminal 10His tag) was expressed CHO cells.
Molecular MassThe 230 amino acid recombinant protein has a predicted molecular mass of 26 kD. The DTT-reduced protein migrates at approximately 39 kD and the non-reduced protein with slightly greater mobility by SDS-PAGE.
Purity>98%, as determined by Coomassie stained SDS-PAGE.
Formulation0.22 µm filtered protein solution is in 10 mM Sodium Phosphate buffer, 500 mM NaCl, pH 7.4.
Endotoxin LevelLess than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration10 and 25 µg sizes are bottled at 100 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & HandlingUnopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
ActivityED50 =10 - 50 ng/ml, corresponding to a specific activity of 0.2 - 1 x 105 units/mg, as determined by M-NFS-60 cell proliferation induction in a dose dependent manner.
ApplicationBioassay
Application NotesBioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Product CitationsBai Q, et al. 2024. Nat Commun. 8195:15. PubMed Phadke L, et al. 2022. Front Aging Neurosci. 14:1056067. PubMed Nakamura T, et al. 2022. J Gynecol Oncol. :. PubMed Zuroff LR, et al. 2020. Front Immunol. 1449:11. PubMed Aktories P, et al. 2022. Cell Rep Methods. 2:100260. PubMed Pasciuto E, et al. 2020. Cell. 182:625. PubMed
StructureHomodimer
DistributionIL-34 mRNA is expressed in different tissues, including spleen, heart, brain, lung, liver, kidney, thymus, testes, ovary, small intestine, prostate, and colon.
FunctionIL-34 increases monocytes viability, induces macrophage proliferation, and synergized with other cytokines to generate macrophages and osteoclasts from cultured progenitors. IL-34 promotes the formation of the colony-forming unit-macrophage (CFU-M) in human bone marrow culture. IL-34 supports RANKL-induced osteoclastogenesis by promoting the adhesion and proliferation of osteoclast progenitors. IL-1β and TNF-α induce the expression of interleukin-34 mRNA in osteoblasts. IL-34 is induced in macrophages infected with equine infectious anemia virus (EIAV).
InteractionMacrophages, monocytes, and myeloid cells
Ligand/ReceptorCSF-1R
Biology AreaImmunology, Innate Immunity, Stem Cells
Molecular FamilyCytokines/Chemokines
Antigen References1. Lin H, et al. 2008. Sciences 320:807. 2. Chihara T, et al. 2010. Cell Death Differ. 17:1917. 3. Wei S, et al. 2010. J. Leukoc. Biol. 88:495. 4. Eda H, et al. 2010. Reumathology PMID:21181166. 5. Covaleda L, et al. 2010. Virology 397:217.
Gene ID762577
UniProtView information about IL-34 on UniProt.org
Regulatory StatusRUO
Other NamesC16orf77 homolog
Biolegend 577602 Recombinant Mouse IL-34 (carrier-free) | IRIGHT