Biolegend, 589302, Recombinant Mouse CCL25 (TECK) (carrier-free)
10microg
Catalog Number: 589302
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| Source | Mouse CCL25, amino acids Gln24-Asn144 (Accession# O35903.2) was expressed in E. coli. |
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| Molecular Mass | The 121 amino acid recombinant protein has a predicted molecular mass of approximately 16 kD. The DTT-reduced and non-reduced protein migrate at approximately 16 kD by SDS-PAGE. The N-terminal amino acid is Glutamine. |
| Purity | >98%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS. |
| Endotoxin Level | Less than 0.01 ng per µg cytokine as determined by the LAL method. |
| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | Bioactivity was measured by its ability to chemoattract Baf3-hCCR9 cells in a dose dependent manner. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | CC Chemokine |
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| Distribution | CCL25 is expressed by small intestinal epithelial cells, thymic medullary dendritic cells, and cortex epithelial cells. |
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| Function | CCL25 is a chemokine that is involved in T cell development in the thymus and in recruiting effector lymphocytes to small intestinal epithelium. CCL25 induces extravasation of IgA antibody-secreting cells in the small intestine. |
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| Interaction | CD4 and CD8 single positive thymocytes, immature double positive thymocytes, macrophages, dendritic cells, IgA antibody-secreting cells, and cancer cells. |
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| Ligand/Receptor | CCR9 |
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| Cell Type | B cells, Dendritic cells, Tregs |
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| Biology Area | Cell Biology, Immunology, Signal Transduction |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Hieshima K, et al. 2004. J. Immunol. 173:3668. |
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| Gene ID | 20300 |
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| UniProt | View information about CCL25 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | TECK (Thymus-Expressed Chemokine), Small-inducible cytokine A25 (SCYA25), CKb15 (Ck beta-15) |
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