Biolegend, 653003, PE anti-NF-κB p65 Antibody
25tests
Catalog Number: 653003
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Partial human NF-κB p65 recombinant protein (451-551 aa) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. |
| Excitation Laser | Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
| Application Notes |
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| This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). |
| RRID | AB_2562768 (BioLegend Cat. No. 653003) AB_2562769 (BioLegend Cat. No. 653004) |
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| Structure | 65 kD protein containing a Rel homology domain (RHD), an activation domain, and a 9aaTAD domain. |
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| Distribution | The inactivated NF-κB complex containing p65 subunit is bound to IκB and is localized to cytoplasm. Upon activation, I-κB is phosphorylated and degraded. The activated NF-κB complex is in turn translocated to the nucleus as a transcription factor. |
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| Function | NF-κB is a homodimeric or heterodimeric complex formed by the Rel-like domain-containing proteins. The most abundant form is p65 (RelA) - p50 (NF-κB1) heterodimer complex. The NF-κB complex is a ubiquitously expressed transcription factor which is involved in various biological functions, such as cell growth, tumorigenesis, differentiation, apoptosis, inflammation, and immune responses. |
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| Interaction | Interacts with NF-κB1 (p105/p50) or NF-κB2 (p100/p52) to form heterodimeric NF-κB complex. Interacts with HDAC1, HDAC3, and CBP. Interaction with MEN1 inhibits transactivation activity of NF-κB complex. |
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| Cell Type | B cells |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors |
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| Molecular Family | Nuclear Markers |
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| Antigen References | 1. Li Z, et al. 1997. Mol. Cell. Biol. 17:6184. 2. Saccani S, et al. 2004. J. Exp. Med. 200:107. 3. Nolan GP, et al. 1991. Cell 64:961. 4. Chen LF, et al. 2001. Science 293:1653. 5. Hansen SK, et al. 1994. Mol. Cell. Biol. 14:2593. 6. Chapman NR, et al. 2002. Biochem J. 366:459. |
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| Gene ID | 4790 |
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| UniProt | View information about NF-kappaB p65 on UniProt.org |
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| Clone | 14G10A21 |
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| Regulatory Status | RUO |
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| Other Names | Nuclear factor NF-kappa-B p65 subunit (NF-κB p65), Rel-A, transcription factor p65, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (NFKB3) |
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| Isotype | Mouse IgG2b, κ |
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