RFQ
Menu
Description
Ephrins are cell surface molecules with a wide variety of biological functions since they are expressed on nearly every tissue of a developing embryo while also persisting throughout adulthood. The ephrin family of ligands consists of five GPI-linked members (ephrin-A1 through A5) in addition to three transmembrane proteins (ephrin-B1 through B3). The interaction between ephrin ligands and their Eph receptors generates bidirectional signaling, affecting both receptor and ligand expressing cells, and contributes greatly to the regulation of neural crest migration, growth cone development, and cancer progression. Mature Ephrin-B2 is comprised of a 202 amino acid extracellular domain, a 21 amino acid transmembrane portion, and an 83 amino acid cytoplasmic domain. It is expressed on presynaptic neurons where it promotes presynaptic development, EphB2 shedding, and axonal guidance. Ephrin-B2 controls VEGF-induced angiogenesis and lymph angiogenesis by vascular mural cells as well as arterial and lymphatic endothelium. It has also been shown to prevent the effects of NMDA receptor (NMDAR) antibodies on neuroplasticity and memory meant to treat NMDAR encephalitis. Ephrin-B2 overexpression occurs in many cancers where it promotes the metastasis, proliferation, and angiogenesis of tumors. Abnormal Ephrin-B2 upregulation has been characterized in glioblastoma, neuroblastoma, oral squamous cell carcinoma, mutant p53 colorectal carcinoma, and gastric cancers.
Specifications

General

SourceMouse Ephrin B2, amino acid (Arg29-Ala229) (Accession: # P52800.1), with a linker and a C-terminal human IgG (Pro100-Lys330), was expressed in CHO cells.
Molecular MassThe 434 amino acid recombinant protein has a predicted molecular mass of approximately 48.13 kD. The DTT-reduced and non-reduced protein shall migrate at approximately 50 - 80 kD and 80 - 120kD by SDS-PAGE. The predicted N-terminal amino acid is Arg.
Purity> 95%, as determined by Coomassie-stained SDS-PAGE
Formulation0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin LevelLess than 0.1 EU per µg cytokine as determined by the LAL method
Concentration25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & HandlingUnopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
ActivityWhen Recombinant Mouse Ephrin-B2 is immobilized at 2 µg/mL, Biotinylated Recombinant Human EphB2 (Cat. No. 791704) binds in a dose-dependent manner with an ED50 range of 1.5 – 9 ng/mL. HRP-Avidin (Cat. No. 405103) was used to detect the binding.
ApplicationBioassay
Application NotesBioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
StructureDimer
DistributionEphrin-B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium
FunctionCell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development.
InteractionNeurons, Endothelial cells
Ligand/ReceptorEphA4, EphA3, EphB2, and EphB4
BioactivityBiotinylated Recombinant Human EphB2 binding to Immobilized Recombinant Mouse Ephrin-B2 in a dose-dependent manner. ED50 of this effect is 1.5 – 9 ng/mL.
Cell TypeEndothelial cells, Neurons
Biology AreaAngiogenesis, Cell Motility/Cytoskeleton/Structure, Neuroscience, Synaptic Biology
Molecular FamilySoluble Receptors
Antigen ReferencesPasquale EB. 2008. Cell. 133:38-52. Wilkinson DG. 2001. Nat Rev Neurosci. 2:155-64. Lin KT, et al. 2008. J Biol Chem. 283:28969-79. Foo SS, et al. 2006. Cell. 124:161-73. Treffy RW, et al. 2016. Dev Neurosci. 38:124-38. Poliak S, et al. 2015. ELife. e10841. Pozniak PD, et al. 2016. J Cell Physiol. 231:1237-48. Wang Y, et al. 2010. Nature. 465:483-6. Benjamin K, et al. 2016. ELife. e14845. Sasabe E, et al. 2017. PLoS One. 11:e0188965. Yue WJ, et al. 2018. Oncol Lett. 16:4455-4461. Thomas MG, et al. 2013. Cell Death Dis. 4:e669. Planagumà J, et al. 2016. Ann Neurol. 80:388-400.
Gene ID13642
UniProtView information about Ephrin-B2 on UniProt.org
Regulatory StatusRUO
Other NamesHTK Ligand, Ephrin B2, EFNB2, Eph-Related Receptor Tyrosine Kinase Ligand 5, Ligand Of Eph-Related Kinase 5, EPLG5, LERK5, HTKL