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Description
CD56, also known as neural cell adhesion molecule 1 (NCAM-1), belongs to the cell adhesion molecules (CAMs) of the immunoglobulin superfamily (IgSF-CAMs). The structure of NCAM consists of five immunoglobulin-like (Ig) domains followed by two fibronectin type III (FNIII) domains, playing a crucial role in neuronal development and synaptic plasticity. The Ig domains of CD56 are involved in homophilic binding, promoting cell-cell adhesion, and assisting in synaptic formation and maintenance. The FNIII domains participate in signaling to induce neurite outgrowth. CD56 also induces neuronal differentiation by activating several signaling molecules. Alterations in CD56 expression and CD56-mediated signaling have been linked to cognitive deficits, such as schizophrenia and Alzheimer's disease. Additionally, studies have shown a positive correlation between CD56 and Hereditary Motor Sensory Neuropathy (CMT). In acute myeloid leukemia (AML), CD56 (NCAM1) has been identified as a biomarker, where its abnormal expression activates the MAPK pathway. CD56 is a significant protein involved in various neural processes, and its dysregulation has implications in several neurological conditions and certain cancers.
Specifications

General

SourceHuman CD56, amino acid (Leu20-Pro603) (Accession # P13591-3), with a C-terminal His tag, was expressed in 293E.
Molecular MassThe 590 amino acid recombinant protein has a predicted molecular mass of approximately 65.51 kD. The DTT-reduced and non-reduced protein shall migrate at approximately 70 -115 kD by SDS-PAGE. The predicted N-terminal amino acid is Leu.
Purity> 95%, as determined by Coomassie-stained SDS-PAGE
Formulation0.22 µm filtered protein solution is in PBS, 2 mM DTT, pH 7.4
Endotoxin LevelLess than 0.1 EU per µg cytokine as determined by the LAL method
Concentration25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & HandlingUnopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
ActivityImmobilized Recombinant Human CD56 supports the adhesion of Neuro-2A cells in a dose-dependent manner with an ED50 range of 0.2 – 1.6 µg/mL.
ApplicationBiossay
Application NotesBioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
StructureMonomer
DistributionNK cells, T subset, neural tissue, some LGL and myeloid leukemias
FunctionInduce neurite outgrowth, neuronal differentiation, and help in the formation and stabilization of synapses between neurons
InteractionFibroblast growth factor receptors (FGFRs)
Ligand/ReceptorMDK (Midkine receptor)
BioactivityHuman CD56 supports the adhesion of N2A cells in a dose-dependent manner.
Cell TypeB cells, Leukemia, Mesenchymal Stem Cells, Neurons, T cells
Biology AreaCell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular FamilyAdhesion Molecules, CD Molecules
Antigen ReferencesKim W, et al.2021. J Biol Chem. 296:100372. Ditlevsen DK, et al. 2008. J Neurosci Res. 86:727-43. Sytnyk V, et al. 2017.Trends Neurosci. 40:295-308. Weledji EP and Assob JC. 2014. Ann Med Surg (Lond). 3:77-81. Berridge MJ. 2013. Prion. 7:2-13. Chu C, et al. 2018. J Cell Biol. 43:1714. Muramatsu H, et al. 2000. Biochem Biophys Res Commun. 270:936-41. Kos FJ and Chin CS. 2002. Immunol Cell Biol. 80:364-9. Jennings MJ, et al. 2022. Brain. 145:3999-4015. Sasca D, et al. 2019. Blood. 133:2305-2319.
Gene ID4684
UniProtView information about CD56/NCAM-1 on UniProt.org
Regulatory StatusRUO
Other NamesNeural Cell Adhesion Molecule 1, NCAM, CD56, NCAM-1, CD56 antigen