Description
Corning Matrigel Matrix for Organoid Culture is a solubilized basement membrane preparation that has been optimized for organoid culture. Verified to support both mouse and human organoid growth and differentiation from healthy and/or diseased tissue, each lot has a specific elastic modulus value indicating the stiffness of the gel formed and is qualified to form stable 3D domes commonly used in organoid culture protocols. Available in 10mL LDEV-free, Phenol Red-free format. Corning Matrigel Matrix for Organoid culture is extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors. Quality Tested for ability to form and maintain stable 3D domes/droplets on multi-well plates. Elastic Modulus (matrix stiffness) measured using a rheometer. Protein concentrations determined by Lowry method Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing Extensive PCR testing is performed to screen for a number of pathogens Tested and found negative for bacteria, fungi, and mycoplasma Endotoxin units measured by Limulus Amoebocyte Lysate assay Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
