Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
DOG1/Anoctamin 1 (F9W4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total anoctamin 1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human anoctamin 1 protein.
Background
Anoctamin 1 is a plasma membrane calcium-activated chloride channel, also known as discovered on gastrointestinal stromal tumors protein 1 (DOG1) or transmembrane protein 16A (TMEM16A) (1). It is essential for chloride secretion from epithelial tissues (2) and has been shown to be a specific marker for cells of Cajal (3), the pacemaker cells that control smooth muscle contraction. Anoctamin 1 knockout mice exhibit an altered gastric smooth muscle rhythmic contraction (4). More recently, research studies have identified anoctamin 1 as a heat sensor in nociceptive neurons (5). Heat above 44ºC triggers anoctamin 1-dependent depolarization, contributing to the mediation of thermal nociception (5).
Alternate Names
ANO1; anoctamin 1; anoctamin 1, calcium activated chloride channel; Anoctamin-1; Ca2+-activated Cl- channel; calcium activated chloride channel; Discovered on gastrointestinal stromal tumors protein 1; DOG1; FLJ10261; oral cancer overexpressed 2; Oral cancer overexpressed protein 2; ORAOV2; TAOS2; TMEM16A; Transmembrane protein 16A; transmembrane protein 16A (eight membrane-spanning domains); tumor amplified and overexpressed sequence 2; Tumor-amplified and overexpressed sequence 2
