Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
SFPQ (F5I3D) Mouse Monoclonal Antibody recognizes endogenous levels of total SFPQ protein. This antibody detects isoforms 1 and 2 of SFPQ. This antibody detects 18 kDa and 30 kDa proteins of unknown identity in some cell lines.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human SFPQ protein.
Background
Splicing factor proline-glutamine rich (SFPQ), also commonly referred to as PTB-associated splicing factor (PSF), is an essential and ubiquitous nucleic acid-binding protein that is involved in a wide range of cellular processes. SFPQ belongs to the behavior/human splicing (DBHS) protein family along with paraspeckle protein 1 (PSPC1) and non-POU domain-containing octamer-binding protein (NONO) (1). DBHS proteins are a unique family of RNA-binding proteins that recruit various proteins and nucleic acids involved in transcriptional activation and repression, transcript localization, splicing, and DNA damage repair (2). SFPQ and NONO are also core components of paraspeckles, which are subnuclear bodies distinct from nuclear speckles that are defined by the colocalization of SFPQ, NONO, and other proteins with the long noncoding RNA NEAT1 (2). The biological function of paraspeckles is currently unknown, but they are believed to be important for gene regulation in the presence of cellular stressors (3). Although SFPQ is typically localized to the nucleus, multiple studies indicate that cytoplasmic SFPQ levels are important for proper motor neuron differentiation and maintenance (4,5). has also been linked to multiple neurological disorders, including Alzheimer's disease (AD) and amyotrophic lateral sclerosis (ALS) (6-9).
Alternate Names
100 kDa DNA-pairing protein; DNA-binding p52/p100 complex, 100 kDa subunit; epididymis secretory sperm binding protein; hPOMp100; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; Polypyrimidine tract-binding protein-associated-splicing factor; POMP100; PPP1R140; protein phosphatase 1, regulatory subunit 140; PSF; PTB-associated splicing factor; PTB-associated-splicing factor; SFPQ; splicing factor proline and glutamine rich; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated); splicing factor proline/glutamine-rich; splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated); Splicing factor, proline- and glutamine-rich
