Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
ITM2B/Bri2 (E6O3Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total human ITM2B/Bri2 protein. Bands at 84 kDa and 21 kDa are occasionally detected, likely representing oligomers of ITM2B/Bri2 and cleavage products, respectively. This antibody does not cross-react with rodent orthologs of ITM2B/Bri2 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala105 of human ITM2B/Bri2 protein.
Background
Integral membrane protein 2b (ITM2B), also known as Bri2, is a type II membrane protein. ITM2B is expressed as a precursor immature form and is processed by furin proteases to produce the mature ITM2B/Bri2 protein and a soluble C-terminal fragment (1,2). The membrane-bound ITM2B/Bri2 protein can be further processed by ADAM10 and intramembrane proteases (1). Several studies have implicated the gene with familial forms of dementia and neurodegenerative diseases, including Alzheimer's disease (AD). Mutations in the human gene are linked to several familial British and Danish dementia disorders (3,4). gene mutations cause abnormal processing of the ITM2B/Bri2 protein, suggesting that the products of ITM2B/Bri2 protein cleavage might contribute directly to disease etiology (4). Interestingly, cleavage of disease-linked mutant -encoded proteins generates peptides (ABri and ADan) that are more prone to deposit as amyloid fibrils, a pathological hallmark of many neurodegenerative diseases (2,4). Additionally, ITM2B/Bri2 interacts with Aβ-precursor protein (APP), a gene/protein linked to AD, and may alter APP processing and fibril formation (5-7). ITM2B/Bri2 is one of a family of proteins containing a BRICHOS domain, identified by their ability to inhibit Aβ fibril formation (8). The specific function of ITM2B/Bri2 is unclear, but it may contribute to normal synaptic function via an unknown mechanism (9).
Alternate Names
ABRI; ABri/ADan amyloid peptide; ABri23; BRI; BRI2; BRI2 ICD; BRI2 intracellular domain; Bri2-23; BRI2, membrane form; Bri23 peptide; BRI2C, soluble form; BRICD2B; BRICHOS domain containing 2B; C-terminal peptide; E25B; E3-16; epididymis secretory sperm binding protein; FBD; imBRI2; Immature BRI2; Integral membrane protein 2B; ITM2B; Mature BRI2; mBRI2; P23 peptide; Protein E25B; RDGCA; Transmembrane protein BRI
