Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
NUFIP2 (F5O7H) Rabbit Monoclonal Antibody recognizes endogenous levels of total NUFIP2 protein. This antibody also reacts with a protein at 65 kDa, which likely represents post-translational modification of NUFIP2.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NUFIP2 protein.
Background
Nuclear FMR1-interacting protein 2 (NUFIP2) was initially identified and characterized as a novel RNA-binding protein associated with the fragile X mental retardation protein (FMRP) (1). NUFIP2 functions as a ribonucleoprotein large subunit assembly factor and facilitates ribosome biogenesis through binding of specific components necessary for protein synthesis (1). As one of many FMRP-interacting proteins, NUFIP2 localizes to several subcellular compartments, including the cytoplasmic stress granule, nuclear body, and ribosome, indicating involvement in both nuclear and cytoplasmic processes (2). Moreover, the subcellular distribution of NUFIP2 is cell cycle-dependent in cultured cells, suggesting that the composition of FMRP-containing RNP complexes may be cell cycle-modulated (1). Under lysosomal-damaging conditions, NUFIP2 directly interacts with GABARAPs of the ATG8 family. Subsequent Atg8ylation of NUFIP2 drives its recruitment to stress granules where it contributes to mTOR inactivation via the Ragulator-RagA/B complex (3,4). NUFIP2 has also been identified as a regulatory cofactor of Roquin-1 and Roquin-2, RNA-binding proteins which recognize secondary structures in target mRNA 3â²-UTRs and induce mRNA decay (5).
Alternate Names
182-FIP; 82 kDa FMRP-interacting protein; 82-FIP; 82-kD FMRP Interacting Protein; Cell proliferation-inducing gene 1 protein; FIP-82; FLJ10976; FMR1-interacting protein NUFIP2; FMRP-interacting protein 2; Fragile X Mental Retardation Protein; KIAA1321; MGC117262; nuclear FMR1 interacting protein 2; nuclear fragile X mental retardation protein interacting protein 2; Nuclear fragile X mental retardation-interacting protein 2; NUFIP2; NUFIP2, FMR1 interacting protein 2; NUFP2; PIG1; proliferation-inducing gene 1
