Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
SENP1 (D16D7) Rabbit Monoclonal Antibody recognizes endogenous levels of total SENP1 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln175 of human SENP1 protein.
Background
SENP1 is a member of the sentrin/SUMO-specific protease (SENP) family. SENP1 localizes to the nucleoplasm and catalyzes the release of SUMO1, SUMO2, and SUMO3 monomers from sumoylated substrates (1,2). SENP1 has been reported to be responsible for intracellular SUMO homeostasis in the control of normal cellular function (2). The removal of sumoylation by SENP1 from many important target proteins, such as HDAC1, HIF-1α, Stat5, p300, Elk-1, and SirT1, leads to the regulation of the related biological pathways (3-8). SENP1-induced desumoylation of HIF-1α stabilizes the target during hypoxia (5), activating downstream VEGF expression and angiogenesis (9). SENP1 desumoylates Stat5 and contributes to Stat5 acetylation and subsequent signaling during normal lymphocyte development (6). Under stress conditions, SENP1 interacts with and inactivates SirT1 by desumoylation, protecting cells from apoptosis (8). SENP1 has been reported to target the progesterone and androgen receptors, either directly or indirectly through HDAC1, thereby upregulating their transcriptional function and potentially affecting receptor-related cancer progression (3,10-13).
Alternate Names
SENP1; Sentrin-specific protease 1; Sentrin/SUMO-specific protease SENP1; SUMO specific peptidase 1; SUMO1/sentrin specific peptidase 1; SUMO1/sentrin specific protease 1; SuPr-2
