Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
PiT1/SLC20A1 (D1Z4X) Rabbit Monoclonal Antibody recognizes endogenous levels of total PiT1 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly290 of human PiT1 protein.
Background
Phosphate transporter 1 (PiT1/SLC20A1) is a sodium dependent phosphate (Pi) transporter that imports Pi into cells. PiT1 was initially identified as a receptor for retroviruses (1,2). It is widely expressed in various tissues where it plays a critical role in maintaining cellular Pi homeostasis (3,4). Phosphate transporter 1 is important in cell proliferation and tumor cell growth independent of PiT1 phosphate transport function (5). Researchers have found that PiT1 is involved in TNF-α induced apoptosis (6). Moreover, phosphate uptake via PiT1 is crucial for vascular calcification (7) and overexpression of PiT1 leads to soft tissue calcification in Werner syndrome patients (8). Additional research indicates that increased PiT1 expression is seen in calcific aortic valve disease (CAVD) tissues, and that PiT1 enhances apoptosis and mineralization by modifying Akt1 levels (9).
Alternate Names
DKFZp686J2397; FLJ41426; Gibbon ape leukemia virus receptor 1; GLVR-1; GLVR1; Leukemia virus receptor 1 homolog; Phosphate transporter 1; PiT-1; PIT1; S20A1; SLC20A1; Sodium-dependent phosphate transporter 1; solute carrier family 20 (phosphate transporter), member 1; Solute carrier family 20 member 1
