Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Immunocytochemistry): 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
TPX2 (D9Y1V) Rabbit Monoclonal Antibody recognizes endogenous levels of total TPX2 protein.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TPX2 protein.
Background
The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport (1,2). TPX2, a target of active Ran (RanGTP), is a microtubule nucleating protein. TPX2 is inactive when bound to nuclear importin-alpha. RanGTP activity disrupts this interaction, relieving inhibition of TPX2 (3). TPX2 binding activates Aurora A kinase and promotes its localization to the mitotic spindle (4,5). DNA damage in mitosis leads to loss of interaction between Aurora A and TPX2 and inactivation of Aurora A kinase (6). TPX2 is highly expressed in pancreatic cancer cells, and knockdown of TPX2 expression in these cells is associated with increased sensitivity to paclitaxel (7).
Alternate Names
C20orf1; C20orf2; Differentially expressed in cancerous and non-cancerous lung cells 2; differentially expressed in lung cells; DIL-2; DIL2; FLS353; GD:C20orf1; HCA519; HCTP4; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; p100; preferentially expressed in colorectal cancer; Protein fls353; REPP86; restricted expression proliferation associated protein 100; Restricted expression proliferation-associated protein 100; Targeting protein for Xklp2; TPX2; TPX2 microtubule nucleation factor; TPX2, microtubule-associated protein homolog; TPX2, microtubule-associated, homolog; TPX2, microtubule-associated, homolog (Xenopus laevis)
