Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
VAMP1 Antibody recognizes endogenous levels of total VAMP1 protein. This antibody does not cross-react with VAMP2.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human VAMP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Vesicle-associated membrane protein 1 (VAMP1), also called synaptobrevin 1, is part of the R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex (1). The SNARE complex is involved in calcium regulated vesicular transport and membrane fusion (2). While related protein VAMP2 exhibits a wider distribution and is more abundant in the brain, VAMP1 is the main isoform in specific brain regions including the subthalamus nucleus zona incerta (1), the ostral periolivary region, and the retina (3). In addition, VAMP1 is involved in neurotransmitter release at the neuromuscular junction (4) and in the release of bioactive peptides from cardiac myocytes (5).
Alternate Names
CMS25; DKFZp686H12131; SPAX1; SYB1; synaptobrevin 1; Synaptobrevin-1; VAMP-1; VAMP1; vesicle associated membrane protein 1; Vesicle-associated membrane protein 1; vesicle-associated membrane protein 1 (synaptobrevin 1)
