Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
S100A6 (D3H3W) Rabbit Monoclonal Antibody recognizes endogenous levels of total S100A6 protein. This antibody also cross-reacts with unidentified proteins at 40 kDa and 60 kDa.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp65 of human S100A6 protein.
Background
Despite their relatively small size (8-12 kDa) and uncomplicated architecture, S100 proteins regulate a variety of cellular processes, such as cell growth and motility, cell cycle progression, transcription, and differentiation. To date, 25 members have been identified, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z, making it the largest group in the EF-hand, calcium-binding protein family. Interestingly, 14 S100 genes are clustered on human chromosome 1q21, a region of genomic instability. Research studies have demonstrated that significant correlation exists between aberrant S100 protein expression and cancer progression. S100 proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development (1-4). Each S100 monomer bears two EF-hand motifs and can bind up to two molecules of calcium (or other divalent cations in some instances). Structural evidence shows that S100 proteins form antiparallel homo- or heterodimers that coordinate binding partner proximity in a calcium-dependent (and sometimes calcium-independent) manner. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellular compartments, and display unique protein binding partners, which suggests that each plays a specific role in various signaling pathways. In addition to an intracellular role, some S100 proteins have been shown to act as receptors for extracellular ligands or are secreted and exhibit cytokine-like activities (1-4). S100A6 (calcyclin) is involved in a number of cellular processes, including exocytosis (5) and cell cycle regulation (6). In addition, S100A6 interacts with a number of proteins such as SIP, GAPDH, and annexins in a calcium-dependent fashion (7,8). Research studies demonstrate that a down regulation of corresponding S100A6 gene expression causes a decrease in cell proliferation (6).
Alternate Names
2A9; 5B10; CABP; CACY; Calcyclin; Growth factor-inducible protein 2A9; MLN 4; PRA; Prolactin receptor-associated protein; Protein S100-A6; S100 calcium binding protein A6; S100 calcium-binding protein A6; S100 calcium-binding protein A6 (calcyclin); S100A6; S10A6
