Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
ThPOK (D9V5T) Rabbit Monoclonal Antibody recognizes endogenous levels of total ThPOK protein. This antibody cross-reacts with a 40 kDa protein of unknown origin.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human ThPOK protein.
Background
The transcription factor Th-inducing POZ/Krüppel-like factor (ThPOK, ZBTB7B, cKROX, ZFP67) is a transcriptional repressor belonging to the POK/ZBTB family of lymphoid cell development regulators (1). ThPOK is best known as a signature CD4+ T cell transcription factor that is upregulated during the differentiation of CD4+ T but not CD8+ cytotoxic T cells (1). Expression of ThPOK in developing T cells represses expression of CD8 and cytotoxic T cell effector genes, and indirectly promotes expression of CD4 by antagonizing RUNX-mediated CD4 repression (2-4). ThPOK expression has also been observed in NKT cells and γδ T cells (5,6).
Alternate Names
c-Krox; CKROX; DKFZp686G01254; hcKrox; Krueppel-related zinc finger protein cKrox; kruppel-related zinc finger protein hcKrox; T-helper-inducing POZ/Krueppel-like factor; THPOK; ZBT7B; ZBTB15; ZBTB7B; Zfp-67; ZFP67; zinc finger and BTB domain containing 15; zinc finger and BTB domain containing 7B; Zinc finger and BTB domain-containing protein 15; Zinc finger and BTB domain-containing protein 7B; Zinc finger protein 67 homolog; Zinc finger protein 857B; Zinc finger protein Th-POK; ZNF857B
