Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
ACAT2 (E1L8V) Rabbit Monoclonal Antibody recognizes endogenous levels of total ACAT2 protein.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val216 of human ACAT2 protein.
Background
Acetyl-CoA acetyltransferase 2 (ACAT2), also known as cytosolic acetoacetyl-CoA thiolase, plays a role in regulating lipid metabolism (1). It catalyzes the synthesis of acetoacetyl-CoA from two acetyl-CoA molecules, which is later converted into steroids (2).
Alternate Names
ACAT2; acetoacetyl Coenzyme A thiolase; acetyl-CoA acetyltransferase 2; Acetyl-CoA acetyltransferase, cytosolic; Acetyl-CoA transferase-like protein; acetyl-Coenzyme A acetyltransferase 2; ACTL; Cytosolic acetoacetyl-CoA thiolase; THIC
