Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Semaphorin 3B (D5A2P) Rabbit Monoclonal Antibody recognizes endogenous level of total semaphorin 3B protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln717 of human semaphorin 3B protein.
Background
Semaphorins are a family of cell surface and secreted proteins initially recognized as axon guidance factors that control the development of the central nervous system (1). They are involved in cell migration, angiogenesis, and immune responses (2-6). Based on protein structure, there are eight classes of semaphorins. Class 3-7 semaphorins are expressed in vertebrates. Semaphorin 3 subfamily members are the only secreted semaphorins in vertebrates. There are seven semaphorin 3 proteins and their receptors include neuropilins and the type A/D family plexins (7-9). Semaphorin 3B functions as a tumor suppressor, as research studies have shown that it is deleted or inactivated in lung and breast cancer (10,11). Overexpression of semaphorin 3B inhibits tumor cell proliferation and causes apoptosis (12,13). Semaphorin 3B also inhibits angiogenesis (14). Semaphorin 3B loses its activity upon cleavage by furin-like pro-protein convertases (14).
Alternate Names
LUCA-1; SEM3B; SemA; Sema A(V); sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B; Sema V; SEMA3B; SEMA5; SEMAA; semaphorin 3B; semaphorin A; Semaphorin-3B; Semaphorin-V; semaV
