Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
NRMT (D9D6P) Rabbit Monoclonal Antibody recognizes endogenous levels of total NRMT protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu49 of human NRMT protein.
Background
N-terminal RCC1 methyltransferase (NRMT), formerly known as methyltransferase-like protein 11A (METTL11A), is a member of the methyltransferase 11 family of proteins and is the first α-N-methyltransferase to be discovered in humans (1-3). Amino-terminal methylation of free α-amino groups is a post-translational modification where an initiating Met residue is cleaved and the exposed α-amino group is mono-, di-, or trimethylated by NRMT (4). NRMT methylates proteins containing an amino-terminal Met-X-Pro-Lys motif, where X is an alanine, proline, or serine residue (4). Substrates of NRMT include the Ran guanine nucleotide-exchange factor (RCC1), SET/TAF-1/PHAP-II, retinoblastoma (Rb), and CENP-B (3-6). α-N-methylation of RCC1 is required for efficient binding to chromatin, securing normal bipolar spindle formation and chromosome segregation (3,5). α-N-methylation of CENP-B also appears to regulate CENP-B binding to centromeric DNA (6).
Alternate Names
AD-003; Alpha N-terminal protein methyltransferase 1A; C9orf32; HOMT1A; ME11A; methyltransferase like 11A; Methyltransferase-like protein 11A; METTL11A; N-terminal RCC1 methyltransferase; N-terminal Xaa-Pro-Lys N-methyltransferase 1; N-terminal Xaa-Pro-Lys N-methyltransferase 1, N-terminally processed; NRMT; NRMT1; NTM1A; NTMT1; X-Pro-Lys N-terminal protein methyltransferase 1A
