Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
UBQLN1 (D3T7F) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBQLN1 protein. This antibody does not cross-react with other UBQLN proteins.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBQLN1 protein.
Background
Ubiquilin 1 (UBQLN1) is a ubiquitously expressed, type 2 ubiquitin like (UBL) protein that contains an amino-terminal UBL domain and a carboxy-terminal Ub-associated (UBA) domain (1). Research studies demonstrate that UBQLN1 associates with poly-Ub chains through its UBA domain, while the UBL domain participates in interactions with proteasome subunits. Evidence suggests that UBQLN1 acts as a shuttling factor during endoplasmic-reticulum-associated protein degradation (ERAD) as it transports misfolded, ubiquitinated proteins from the ER to the proteasome for subsequent degradation (2-5). Additional research studies demonstrate that the UBL domain of UBQLN1 binds UIM-containing endocytic proteins and participates in the sequestration of protein aggregates during aggresome formation (6,7). UBQLN1 regulates presenilin protein levels and is localized in neurofibrillary tangles of Alzheimer's disease-affected brains (8). Polymorphisms in the corresponding gene may be associated with a risk of Alzheimer's disease (9-11).
Alternate Names
DA41; DSK2; FLJ90054; hPLIC-1; PLIC-1; PLIC1; Protein linking IAP with cytoskeleton 1; testicular tissue protein Li 219; ubiquilin 1; Ubiquilin-1; UBQL1; UBQLN1; UBQN; XDRP1
