CST, 14747P, SignalStain ® EDTA Unmasking Solution (10X)
Buffer for studying in the research area.
Catalog Number: 14747P
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Category
Description
Product Usage Information
Dilute to a 1X Unmasking Solution using ddH 2 O just before use. Bring slides to a boil in 1X SignalStain ® EDTA Unmasking Solution. Follow with 15 min at a sub-boiling temperature. No cooling is necessary. Antigen unmasking protocols utilizing a water bath are not recommended. While antigen unmasking performed with a microwave is preferred, the staining of particular tissues or antigen targets may require the use of a pressure cooker.
Storage
Store 10X buffer at 4°C. At 10X, this buffer is stable for 12 months. Always prepare fresh 1X solutions daily.
Background
Proteins within fixed tissue sections contain chemical cross-links that may prevent antibody access or mask antigen targets in immunohistochemical assays. This limited access may vary among different tissue or antigens. Heat-induced antigen unmasking improves accessibility of antibodies to tissues or antigens (1).
Alternate Names
cooker; formalin; IHC; IHC-P; IHCP; immunohistochemistry; microwave; paraffin; pressure; retrieval; unmasking
Dilute to a 1X Unmasking Solution using ddH 2 O just before use. Bring slides to a boil in 1X SignalStain ® EDTA Unmasking Solution. Follow with 15 min at a sub-boiling temperature. No cooling is necessary. Antigen unmasking protocols utilizing a water bath are not recommended. While antigen unmasking performed with a microwave is preferred, the staining of particular tissues or antigen targets may require the use of a pressure cooker.
Storage
Store 10X buffer at 4°C. At 10X, this buffer is stable for 12 months. Always prepare fresh 1X solutions daily.
Background
Proteins within fixed tissue sections contain chemical cross-links that may prevent antibody access or mask antigen targets in immunohistochemical assays. This limited access may vary among different tissue or antigens. Heat-induced antigen unmasking improves accessibility of antibodies to tissues or antigens (1).
Alternate Names
cooker; formalin; IHC; IHC-P; IHCP; immunohistochemistry; microwave; paraffin; pressure; retrieval; unmasking
