Description
Product Usage Information
Immunofluorescence (Frozen): 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunofluorescence (Frozen)
Specificity / Sensitivity
Doublecortin (A8L1U) Rabbit Monoclonal Antibody recognizes endogenous levels of total doublecortin protein.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human doublecortin protein.
Background
Mutations in doublecortin ( ) cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule-associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5, and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331, and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).
Alternate Names
DBCN; DC; DCX; doublecortex; doublecortin; Doublin; Lis-X; Lissencephalin-X; LISX; Neuronal migration protein doublecortin; SCLH; XLIS
