Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
AGPAT2 (D8W9B) Rabbit Monoclonal Antibody recognizes endogenous levels of total AGPAT2 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly235 of human AGPAT2 protein.
Background
The 1-acylglycerol-3-phosphate-O-acyltransferase 2 enzyme (AGPAT2) catalyzes the acylation of lysophosphatidic acid (LPA) into phosphatidic acid (PA), which is a precursor for the synthesis of triacylglycerol and phospholipid (1,2). AGPAT2 is highly expressed in adipose tissues, liver, and skeletal muscle (3). The induced knockdown of AGPAT2 expression results in decreased expression of adipogenic proteins and delayed expression of adipogenic marker proteins, suggesting that AGPAT2 plays an important role in adipocyte growth and differentiation (4). Mutations in the corresponding gene cause autosomal recessive congenital generalized lipodystrophy type 1 (CGL1), also described as Berardinelli-Seip syndrome. Patients with CGL1 are born without detectable white adipose tissue and tend to develop severe insulin resistance, hypertriglyceridemia, and type 2 diabetes during childhood (5,6).
Alternate Names
1-acyl-sn-glycerol-3-phosphate acyltransferase beta; 1-acylglycerol-3-phosphate O-acyltransferase 2; 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase-beta); 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta); 1-AGP acyltransferase 2; 1-AGPAT 2; 1-AGPAT2; AGPAT2; BSCL; BSCL1; LPAAB; LPAAT-beta; Lysophosphatidic acid acyltransferase beta; lysophosphatidic acid acyltransferase-beta; PLCB; testicular tissue protein Li 143
