Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:50 - 1:200
Immunofluorescence (Frozen): 1:800
Immunofluorescence (Immunocytochemistry): 1:800
Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 61751 .
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
Cre Recombinase (D7L7L) Rabbit Monoclonal Antibody recognizes transfected and transgenic levels of total Cre recombinase protein.
Species Reactivity: All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of bacteriophage-P1 Cre recombinase protein.
Background
Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34-base-pair loxP sites. This reaction can be carried out , indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of and applications, including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6).
Alternate Names
cre; cre recombinase; cyclization recombinase; Recombinase cre
