Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
IkappaB beta (D1T3Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total IκBβ protein. This antibody is recommended for immunofluorescence in mouse, but not human cells.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse IκBβ protein.
Background
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). The regulation of IκBβ and IκBε is similar to that of IκBα. However, the phosphorylation and ubiquitin-mediated degradation of these proteins occurs with much slower kinetics (9). IKK phosphorylation of IκBβ occurs at Ser19 and Ser23, while IκBε can be phosphorylated at Ser18 and Ser22 (10).
Alternate Names
I-kappa-B-beta; I(Kappa)B(beta); IKapp; IkappaBbeta; IkB; IkB-B; IkB-beta; Ikbb; MGC36057; NF-kappa-B inhibitor beta; NF-kappa-BIB; Nfkbib; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, beta; nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, beta; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta; OTTMUSP00000018308; OTTMUSP00000018309
