Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:100 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
SV40 Large T Antigen (D1E9E) Rabbit Monoclonal Antibody recognizes transgenic levels of total SV40 Large T Antigen protein.
Species Reactivity: Virus
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys315 of SV40 Large T Antigen protein.
Background
Simian Virus 40 (SV40) is a small double-stranded, circular DNA tumor virus encoding three tumor antigens: large T, Small T, and 17KT. These antigens contribute to cellular transformation, an area of research where they are utilized extensively. Transformation of cells by SV40 large T antigen is mediated by its binding to the tumor suppressor proteins RB and p53 in the nucleus. Additional binding partners include p300, CBP, Cul7, IRS1, Bub1, Nbs1, and Fbw7, which also contribute to transformation (1).
Alternate Names
Large T antigen; LT; lT-AG; simian virus 40 large T antigen; SV40 large T antigen
