Description
Product Usage Information
Flow Cytometry (Fixed/Permeabilized): 1:50
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
Phospho-CD79A (Tyr210) (E8E9Z) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr210 of human CD79A protein.
Background
Antigen receptors found on the surface of B cells contain a heterodimeric signaling component composed of CD79A and CD79B, also known as Ig α and Ig β, respectively (1,2). Presence of this receptor complex is essential for B cell development and function (3). Together these two proteins and the associated B cell receptor (BCR) initiate intracellular signaling following antigen binding (4,5). An immunoreceptor tyrosine-based activation motif (ITAM) found in the CD79A intracellular region appears to be important for its function (6). Antigen binding precedes formation of the CD79A and CD79B heterodimer and subsequent activation of receptor associated kinases (7). Research has shown that CD79A is a marker for B-lineage lymphoblastic leukemia (8). Additionally, investigators have found that mutations in the ( ) gene are associated with abnormally low levels of functional B cell receptors in some cases of chronic B cell lymphocytic leukemia (9). Tyrosine 210 is an evolutionarily conserved non-ITAM tyrosine residue that lies within the cytoplasmic domain of CD79A/Ig-α. Research studies have shown that ligation of the BCR induces phosphorylation of tyrosine 210, which facilitates both the recruitment of BLNK to the BCR signaling complex and the activation of downstream Syk-dependent signaling (10,11). Collectively, these molecular events drive B cell activation and proliferation (12).
Alternate Names
B-cell antigen receptor complex-associated protein alpha chain; CD79A; CD79A antigen; CD79a antigen (immunoglobulin-associated alpha); CD79a molecule; CD79a molecule, immunoglobulin-associated alpha; Ig-alpha; IGA; MB-1; MB-1 membrane glycoprotein; MB1; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein
