Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
proBDNF (E6Z7C) Rabbit Monoclonal Antibody recognizes full-length recombinant BDNF and exogenously expressed BDNF. This antibody does not recognize the mature form of BDNF.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse BDNF protein.
Background
Neurotrophins are composed of at least four family members, including NGF, BDNF, NT-3, and NT-4, and all are known to influence growth, development, differentiation, and survival of neurons (1). Proneurotrophins bind to p75NTR, but not to the family of Trk receptor tyrosine kinases (Trk), and following maturation, BDNF binds and activates TrkB. Trk receptors, in turn, activate three major signaling pathways: (a) Ras-MAPK signaling, which promotes neuronal differentiation and neurite outgrowth, (b) PI3K/Akt signaling, which promotes survival and growth of neurons, and (c) PLC-γ1-PKC signaling, which promotes synaptic plasticity (2). BDNF is a major regulator of transmission and plasticity at adult synapses. Moreover, the precursor proBDNF and the mature protein mBDNF drive opposite effects on long-term potentiation and long-term depression (3). BDNF has also been implicated in body weight regulation and activity: heterozygous BDNF knockout mice are hyperphagic, obese, and hyperactive (4).
Alternate Names
anorexia BDNF; BDNF; BDNF precursor form; brain derived neurotrophic factor; Brain-derived neurotrophic factor; OTTMUSP00000016087; OTTMUSP00000016088; OTTMUSP00000016089; OTTMUSP00000016090; OTTMUSP00000016091; OTTMUSP00000016092; OTTMUSP00000016093; OTTMUSP00000016094; OTTMUSP00000016095; OTTMUSP00000016096; ProBDNF
