Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
CD96 (E4B8Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse CD96 protein. This antibody is more sensitive by immunoprecipitation for the unglycosylated form of CD96 protein.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu368 of mouse CD96 protein.
Background
Cluster of Differentiation 96 (CD96), also known as T cell-activated increased late expression protein (TACTILE), is a heavily glycosylated type I transmembrane protein belonging to the immunoglobulin superfamily (1-3). CD96 is expressed on peripheral T cells and natural killer (NK) cells, and is strongly upregulated on these cell types after activation (1,4). It is also expressed on transformed T cells, and at very low levels on activated B cells (1,5,6). CD96 interacts with the receptor ligand PVR (CD155), and is involved in adhesion of activated T and NK cells to target cells during the late phase of the immune response (2,4). CD96 competes with DNAM-1 (CD226) for binding to PVR (CD155) in order to negatively regulate T and NK cell activity, and thus blockade of CD96 in order to induce anti-tumor immune responses is investigated for potential immunotherapeutic intervention in cancer (7-9). Alternative splicing occurs at this locus, and two transcript variants encoding distinct isoforms with variable functions have been identified (1,10).
Alternate Names
1700109I12Rik; Cd96; CD96 antigen; Cell surface antigen CD96; OTTMUSP00000018537; T cell activation, increased late expression; T cell-activated increased late expression protein; T-cell surface protein tactile; TACT; Tactile
