Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
OMG (F8B7X) Rabbit Monoclonal Antibody recognizes endogenous levels of total OMG protein. This antibody detects a 70 kDa protein of unknown identity in some cell lines.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human OMG protein.
Background
Oligodendrocyte-myelin glycoprotein (OMG or OMgp) is a glycosylphosphatidylinositol (GPI)-linked membrane protein expressed in oligodendrocytes and myelin in the central nervous system (CNS) (1,2). OMG is a potent inhibitor of neurite outgrowth expressed in the late stages of myelination (3,4). It has been shown that this inhibitory effect occurs through interaction with Nogo receptor (NgR) and its associated complex; thus, disruption of this interaction may be a target for axonal recovery after injury (5). Altered levels of OMG protein have been seen in cerebrospinal fluid of patients with frontotemporal dementia and Alzheimer's disease, implicating OMG in neurodegenerative diseases (6,7). OMG has also been implicated as a potential autoantigen in CNS inflammatory diseases such as acute disseminated encephalomyelitis and multiple sclerosis (8).
Alternate Names
oligodendrocyte myelin glycoprotein; Oligodendrocyte-myelin glycoprotein; OMG; OMGP
