Description
Product Usage Information
Western Blotting: 1:1000
IHC Leica Bond: 1:100 - 1:400
Immunohistochemistry (Paraffin): 1:200 - 1:800
Immunofluorescence (Frozen): 1:800 - 1:3200
Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200
Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400
Flow Cytometry (Live): 1:100 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 71948 .
Protocol
Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live)
Specificity / Sensitivity
4-1BB/CD137/TNFRSF9 (E2J5H) Rabbit Monoclonal Antibody recognizes endogenous levels of total 4-1BB/CD137/TNFRSF9 protein.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the extracellular domain of mouse 4-1BB/CD137/TNFRSF9 protein.
Background
TNFRSF9 is a member of the tumor necrosis factor receptor superfamily (1,2). It is also called 4-1BB or CD137 (1, 2). 4-1BB/CD137/TNFRSF9 is expressed in activated CD4+ and CD8+ T cells, natural killer cells and dendritic cells (2-5). The ligand 4-1BBL/CD137L/TNFSF9 on antigen presenting cells binds to 4-1BB/CD137/TNFRSF9 and costimulates the activation of T cells (5). The binding of agonistic antibodies to 4-1BB/CD137/TNFRSF9 also leads to costimulation for T cell activation (5). Studies have shown the effectiveness of targeting 4-1BB/CD137/TNFRSF9 by its agonistic antibodies in cancer immunotherapy (6).
Alternate Names
4-1BB; 4-1BB ligand receptor; A930040I11Rik; AA408498; AI325004; Cd137; CD137 antigen; CDw137; Ila; Ly6; Ly63; OTTMUSP00000010903; OTTMUSP00000010904; OTTMUSP00000010905; secreted CD137 antigen; T-cell antigen 4-1BB; Tnfrsf9; TNR9; Tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9
