Description
Product Usage Information
Immunohistochemistry (Paraffin): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 29460 .
Protocol
Available protocols: Immunohistochemistry (Paraffin)
Specificity / Sensitivity
Mast Cell Chymase/CMA1 (F6S4Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total mast cell chymase/CMA1 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr195 of human mast cell chymase/CMA1 protein.
Background
Mast cell chymase, also known as chymase 1 (CMA1), is one of the major proteases expressed and secreted by mast cells (1). CMA1 is found in cytoplasmic granules before it is released. It converts angiotensin I to angiotensin II and is the major enzyme responsible for doing so in the heart (2). CMA1 can promote pathogenesis of chronic obstructive pulmonary disease (COPD) by inducing TNF-α in lung macrophages (3). CMA1 activity is also implicated in fibrosis in various tissues (4). In COVID-19 patients, CMA1-positive mast cells are significantly elevated, and CMA1 levels are associated with disease severity (5,6). In gastric cancer, CMA1 has been proposed as a prognostic marker, with high expression associated with poor overall survival and with high levels of tumor-infiltrating lymphocytes (TILs) (7).
Alternate Names
Alpha-chymase; Chymase; chymase 1; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase 1, mast cell; chymase, heart; chymase, mast cell; CMA1; CYH; CYM; Mast cell protease I; MCT1; MGC119890; MGC119891
