Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
UBASH3B (E1A8Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBASH3B protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UBASH3B protein.
Background
Ubiquitin-associated and SH3 domain-containing protein B (UBASH3B) is a ubiquitously expressed protein that negatively regulates signaling cascades governed by multiple tyrosine kinases. UBASH3B is a modular protein that contains an N-terminal UBA domain that can interact with ubiquitin, a central SH3 domain that mediates protein-protein interactions, and an evolutionarily conserved C-terminal phosphatase domain. Indeed, research studies have shown that UBASH3B interacts with Bcr-Abl and negatively regulates its kinase activity through a mechanism involving dephosphorylation (1). UBASH3B also plays a pivotal role in the adaptive immune response by negatively regulating the activity of key proximal signaling proteins downstream of the T cell receptor (TCR) (2-4). Research studies have also positioned UBASH3B as an oncogenic protein in the context of triple-negative breast cancer, where its overexpression promotes enhanced EGFR stability via protection from c-Cbl-mediated ubiquitination (5,6).
Alternate Names
Cbl-interacting protein p70; Cbl-interacting protein Sts-1; KIAA1959; MGC15437; nm23-phosphorylated unknown substrate; p70; SH3 domain-containing 70 kDa protein, suppressor of T-cell receptor signaling 1, nm23-phosphorylated unknown substrate; STS-1; STS1; Suppressor of T-cell receptor signaling 1; T-cell ubiquitin ligand 2; TULA-2; TULA2; Tyrosine-protein phosphatase STS1/TULA2; UBASH3B; ubiquitin associated and SH3 domain containing B; ubiquitin associated and SH3 domain containing, B; Ubiquitin-associated and SH3 domain-containing protein B; UBS3B
