Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Agrin/AGRN (F1M3D) Rabbit Monoclonal Antibody recognizes endogenous levels of total agrin/AGRN protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp58 of human agrin/AGRN protein.
Background
Agrin is an extracellular matrix heparan sulfate proteoglycan. Alternative splicing within the C-terminus of the protein generates neural and non-neural agrin, which interact with distinct binding partners to engage different signaling cascades (1). Neural agrin plays a crucial role in the formation of skeletal neuromuscular synapses between presynaptic motor neurons and postsynaptic muscle fibers, as well as in synaptic differentiation (2,3), through its interaction with the transmembrane protein LRP4, which leads to acetylcholine receptor (AChR) aggregation via the activation of MuSK (4,5). Agrin binds to α-dystroglycan (DAG1) and promotes cardiac regeneration (6,7). Agrin is upregulated in non-small cell lung cancer, hepatocellular carcinoma, and pancreatic ductal adenocarcinoma. It promotes tumor angiogenesis, EMT, and metastasis by maintaining VEGFR2 stability on the cell membrane, regulating focal adhesions, and activating PI3K/Akt and YAP pathways (8-12).
Alternate Names
Agrin; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 22 kDa fragment; Agrin C-terminal 90 kDa fragment; Agrin N-terminal 110 kDa subunit; agrin proteoglycan; AGRN; C22; C90; CMS8; CMSPPD; FLJ45064
