Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ST14/Matriptase Antibody recognizes endogenous levels of total ST14/Matriptase protein.
Species Reactivity: Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn280 of human ST14/Matriptase protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Suppressor of tumorigenicity 14 protein (ST14), also known as matriptase, is a type II transmembrane protease (1,2). It is highly expressed in epithelia cells and important for the differentiation and homeostasis of epithelium (3). ST14 is synthesized as a single-chain precursor, and cleaved first at Gly149 in endoplasmic reticulum or Golgi. The resulting N-terminal and C-terminal fragments are non-covalently associated, and interact with hepatocyte growth factor activator inhibitor 1 (HAI-1), which facilitates the transportation of ST14 to the plasma membrane. Cell surface ST14 can be activated by autocleavage at Arg614 though an incompletely understood mechanism. HAI-1 inhibits activated ST14, and HAI-1 and ST14 complex can be shed from cell surface (4,5). In different context, ST14 has been reported either as a tumor suppressor or as a tumor promoter (6-8)
Alternate Names
ARCI11; HAI; Matriptase; Membrane-type serine protease 1; MT-SP1; MTSP1; Prostamin; PRSS14; Serine protease 14; Serine protease TADG-15; SNC19; ST14; ST14 transmembrane serine protease matriptase; suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin); suppression of tumorigenicity 14 (colon carcinoma); Suppressor of tumorigenicity 14 protein; TADG15; TMPRSS14; tumor associated differentially expressed gene 15 protein; Tumor-associated differentially-expressed gene 15 protein
