Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
RFX2 (F6S2V) Rabbit mAb recognizes endogenous levels of total RFX2 protein. This antibody is not expected to cross-react with other RFX proteins.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val340 of human RFX2 protein.
Background
Regulatory factor X2 (RFX2) is a DNA-binding protein and a member of the RFX gene family of transcriptional regulators (1-3). RFX2 is expressed in various cells during spermatogenesis, including spermatocytes and spermatids (2,3). RFX2 is also present in cells requiring cilia, such as multi-ciliated epithelial cells (MCC). It acts as a transcription factor to control their development and function, which is essential for fluid flow in many organ systems (4). RFX2 is associated with neutrophil survival during steady state and inflammation (5).
Alternate Names
DNA binding protein RFX2; DNA-binding protein RFX2; FLJ14226; HLA class II regulatory factor RFX2; Regulatory factor X 2; regulatory factor X, 2 (influences HLA class II expression); regulatory factor X2; RFX2; trans-acting regulatory factor 2
