Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ATL3 (F5T5J) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATL3 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly519 of human ATL3 protein.
Background
Atlastin-3 (ATL3) is a dynamin-like, integral membrane GTPase that is localized to the endoplasmic reticulum (ER) membrane and critical for ER morphogenesis and tubular network biogenesis (1-3). ATL3 also functions as a cargo receptor for ER-phagy and promotes tubular ER degradation by specifically binding to GABARAP subfamily proteins. This ATL3-GABARAP interaction is essential for ATL3 to function in ER-phagy (4). Mutant ATL3 proteins are associated with hereditary sensory and autonomic neuropathies, illustrating the dependence of long-term sensory neuronal maintenance on the structural organization of the ER (5,6). Zika and coronavirus have been shown to modulate ER-phagy to drive viral replication by hijacking ATL3 and other ER-phagy receptors (7,8).
Alternate Names
ATL3; ATLA3; atlastin 3; atlastin GTPase 3; Atlastin-3; DKFZp564J0863; HSN1F
