Description
Product Usage Information
Immunohistochemistry (Paraffin): 1:50 - 1:200
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Immunohistochemistry (Paraffin)
Specificity / Sensitivity
CD14 (D1U2Y) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp327 of mouse CD14 protein.
Background
CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage (1). Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein (2). LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection (3-5).
Alternate Names
Cd14; CD14 antigen; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
